Quick fish sampling for disease diagnostics: Wet mount sampling guide (for ectoparasites & fungi)

Parasitic and fungal infestations represent one of the major challenges to sustainable aquaculture. As part of routine health checks or during abnormal mortality events, screening for ectoparasites and fungi in tilapia, carp and catfish production systems is important to minimize the risk of their introduction, transmission and spread.

WorldFish and partners developed this quick guide for ectoparasites and fungi assessment under a light compound microscope. Standard wet-mount specimens consist of gill biopsy (gill clip), fin biopsy (fin clip) and skin scraping (mucus smears). Additional smears may be included in the presence of external lesions/ulcers (eye, skin, mouth).

A free online training course on wet mount sampling is also available via Learn.ink.


Publisher: WorldFish

Rights: Creative Commons Attribution-NonCommercial.


Quick fish sampling for disease diagnostics: Package of practices

Quality biological sampling is a fundamental requirement for all kinds of work on diagnosing diseases and screening for pathogens. Fish sampling requires specific techniques and skills to collect good biological samples for accurate disease diagnosis. This package of practices developed by WorldFish and partners provides advice on fish sampling protocols for routine disease diagnostics and outbreak investigations in tilapia, carp and catfish production systems. These sampling protocols provide guidance and training to project team members, researchers, resident veterinarians, extension officers and farmers tasked with collecting quality biological samples for laboratory diagnosis whenever disease outbreaks occur on farms or hatcheries. A free online introductory course on fish sampling for disease diagnostics is also available via Learn.ink.