Major pathogens of fish and other farmed aquatic animals include bacteria, fungi, viruses and parasites. These can lead to serious infectious diseases and losses representing one of the major limitation to sustainable aquaculture. Routine health checks for key priority pathogens of tilapia, carp and catfish—will vary with geographies—are important to minimize the risk of their introduction, transmission and spread. On suspicion of an unknown disease during abnormal mortalities, it is routine for the investigators to collect clinical samples from moribund fish for molecular and virology diagnostics. Molecular diagnostics are techniques used to amplify small DNA/RNA sequence(s) that are unique to a particular pathogen to ascertain their presence or absence. Virology is a branch of microbiology that study viruses and viral diseases. WorldFish and partners developed this quick fish sampling guide for molecular and virology diagnostics. Standard specimens for general molecular and virology health evaluation include kidney, liver, spleen, brain and gills. Other tissues may be collected.

During investigation of an abnormal mortality event, fish tissues (or biopsies) for histopathology analysis are collected for disease diagnosis. Histology consists in the preparation of thin, stained tissue sections for microscopic examination to study their structure and function. Histopathology is the study of disease and disease processes by looking at the change in the anatomy or anomalies from cells, tissues and organs as seen through a microscope. WorldFish and partners developed this quick fish-sampling guide for histology. Standard biopsy specimens for histological examination consists of fixed sections of brain, gill, heart, intestine, kidney, liver and spleen. Other tissues may be collected in the presence of external lesions/ulcers (e.g., eye, skin, muscle). A free online training course on histology sampling is also available via Learn.ink.

In this issue:

• Second High-Level Meeting on Aquaculture Transformation in Asia and the Pacific Region.
• Reported aquatic animal diseases in the Asia-Pacific region during the second quarter of 2023.
• Expert Workshop on Aquaculture Effluent Management.
• Larvi 2024: First announcement and call for papers.
• Tuskfish CMS 2.0.7 released.

In this issue:

• How welfare assessments of farmed white leg shrimp (Penaeus vannamei) can benefit the whole industry
• Thai Fish Project: A path towards a sustainable aquaculture
• Nationally-recognised ornamental fish breeder Kripan Sarkar - a man to remember
• Tilapia parvovirus disease: An emerging threat for the tilapia aquaculture industry
• Naihati fish seed market as state-of-the-art for sustainable support services to fish growers, buyers and traders
• NACA Newsletter

The Thai Fish Project began in 2019, and involves around 200 research members and multiple institutions in Thailand and Japan. The project aims to promote domestication and wise use of two Thai native aquatic species, namely the Asian sea bass Lates calcarifer and banana shrimp Penaeus merguiensis through increasing productivity, reducing the impact of infectious diseases, and preserving genetic resources. The project also encompasses several specific research topics to ensure that it comprehensively addresses the concerns on safeguarding the food security and enhancing the environmental sustainability as much as possible. This article describes the main research activities, outputs and future expectations of the Thai Fish Project.