This FAO publication on brine shrimp is a manual for all those who are using Artemia or have an interest in this organism, whether as a source of live food in the hatchery, as a model organism in research, or for other purpose. It is intended for those who wish to update their knowledge on its biology, production or its use, but also for those who want to learn about Artemia for the first time. This manual presents in a concise form essential information on Artemia biology and the most important natural cyst resources that find their way to the aquaculture market. It also provides detailed information on general principles and practical procedures to produce Artemia in ponds and in tank systems. Finally, the manual offers a compilation of state-of-the-art guidelines and methodologies related to the use of this crucial live food organism in aquaculture.