This manual provides guidelines for the production of tiger grouper fingerlings. It outlines best-practice methods for broodstock maintenance, spawning, egg incubation and rearing of larvae through to 2–3 cm, fully metamorphosed juveniles. The guidelines have been developed from the outcomes of ACIAR-funded research, as well as from the experience of Indonesian, Philippine and Australian scientists and commercial hatchery operators, and published information. It provides an aid for improving the availability of grouper seed stock.