The Asia Regional Advisory Group on Aquatic Animal Health meets annually to discuss regional health issues including emerging disease threats. This report includes a review of regional disease status circa 2014, global and regional disease reporting arrangements, global issues and standards, progress in implementation of the the Regional Technical Guidelines on Health management for the Responsible Movement of Live Aquatic Animals, identification and designation of regional aquatic animal health resources and regional and international cooperation.

In this issue:

Anti-microbials and alternatives. Successful demonstration of new model for rural development in Myanmar. Optimisation of Nile tilapia (Oreochromis niloticus) production in ponds based on improved farm management practices in Rwanda. EUS infection in freshwater fishes of Andhra Pradesh. Development of pond-reared broodstock / spawners of green mud crab Scylla serrata. ASEAN Gender Network launched. A two-tube nested PCR detection method for AHPND bacteria. 9th Regional Grouper Hatchery Production Training Course.

In this issue:

12th Technical Advisory Committee held in Cha-am, Thailand. Audio recordings: WAS special session on regional cooperation for improved biosecurity. AFSPAN Final Technical Report now available! Pillay Aquaculture Foundation Awards for Scientists in Least Developed Countries. Gender seminar conducted and ASEAN Gender Network launched. A two-tube, nested PCR detection method for AHPND bacteria. 9th Regional Grouper Hatchery Production Training Course. Developing an environmental monitoring system to strengthen fisheries and aquaculture in the Lower Mekong Basin. Asia-Pacific Regional Workshop on the Status of Aquatic Genetic Resources for Food and Agriculture.

In early 2013, Vibrio parahaemolyticus was discovered as the causative agent of acute hepatopancreatic necrosis disease (AHPND). Subjecting 4 arbitrarily selected V. parahaemolyticus isolates obtained from a single farm in November 2012 to the previously reported immersion challenge protocol, we confirmed that 3 caused high mortality at different rates accompanied by characteristic lesions of AHPND while 1 caused high mortality without such lesions. Virulence may be determined by mobile genetic elements such as plasmids or bacteriophages.

A special session on Regional Cooperation for Improved Biosecurity was held at the World Aquaculture Adelaide 2014 conference, from 7-11 June. The session discussed i) regional cooperation in biosecurity, ii) dealing with emerging diseases, focussing on acute hepatopancreatic necrosis disease, and iii) domestication programmes and their implications for genetic diversity, disease susceptibility and resistance.

The emergence and spread of new or previously unknown infectious diseases has had very significant impacts on public health, food security and international trade. Disease emergence is the consequence of environmental and/or sociological changes that disrupt the ecological relationship between pathogens and their natural hosts. Understanding the drivers of disease emergence will help us develop policies and practices that will reduce opportunities for disease emergence in aquaculture and improve prospects of containment.

A new method for the detection of AHPND-bacteria (AP4) has been published and is available for download. The advantage of the AP4 method over the previously published AP3 method is that it has 100 times higher sensitivity. Because of its higher sensitivity, the bacterial culture enrichment step needed when using the AP3 with low levels of AHPND bacteria may be omitted. However, the AP4 method should not be considered as a replacement for AP3.

Further training provided to aquaculturists in Fiji. Spatial planning for sustainable coastal shrimp production. Availability of grouper (Serranidae) fingerlings and seed in the coral reef of Son Tra Peninsula, central Viet Nam. Small-scale carp seed production through portable FRP hatchery at Khanguri, Odisha. Regional consultation on culture-based fisheries developments in Asia. Gender Assessment Synthesis Workshop. Broodstock management in aquaculture. Urgent appeal to control spread of the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP).

In this issue:

Regional consultation on culture-based fisheries developments in Asia. Gender Assessment Synthesis Workshop. NACA participation in the 5th Global Symposium on Gender in Aquaculture and Fisheries, Lucknow, India. Broodstock Management in Aquaculture: Long term effort required for regional capacity building. Urgent appeal to control spread of the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP).

Enterocytozoon hepatopenaei (EHP) is a microsporidian parasite that was first characterized and named from the giant or black tiger shrimp Penaeus monodon from Thailand in 2009. EHP is confined to the shrimp hepatopancreas (HP) and morphologically resembles an unnamed microsporidian previously reported in the HP of Penaeus japonicas from Australia in 2001. Together, these studies suggest that EHP is not an exotic pathogen but that it is endemic to Australasia.