A Regional Proficiency Testing Program for Aquatic Animal Disease Diagnostic Laboratories in Asia-Pacific was developed to strengthen diagnostic capability across Asia. This capability was identified as a requirement to facilitate the sanitary safety of trade in aquatic animal products. The program provided 41 laboratories across the Asia-Pacific with the opportunity to assess their diagnostic performance for 10 regionally significant aquatic animal pathogens, and to adapt or modify practices where necessary to improve.

The Asia Regional Advisory Group on Aquatic Animal Health meets annually to discuss regional health issues including emerging disease threats. This report includes a review of regional disease status circa 2014, global and regional disease reporting arrangements, global issues and standards, progress in implementation of the the Regional Technical Guidelines on Health management for the Responsible Movement of Live Aquatic Animals, identification and designation of regional aquatic animal health resources and regional and international cooperation.

In this issue:

Anti-microbials and alternatives. Successful demonstration of new model for rural development in Myanmar. Optimisation of Nile tilapia (Oreochromis niloticus) production in ponds based on improved farm management practices in Rwanda. EUS infection in freshwater fishes of Andhra Pradesh. Development of pond-reared broodstock / spawners of green mud crab Scylla serrata. ASEAN Gender Network launched. A two-tube nested PCR detection method for AHPND bacteria. 9th Regional Grouper Hatchery Production Training Course.

In this issue:

12th Technical Advisory Committee held in Cha-am, Thailand. Audio recordings: WAS special session on regional cooperation for improved biosecurity. AFSPAN Final Technical Report now available! Pillay Aquaculture Foundation Awards for Scientists in Least Developed Countries. Gender seminar conducted and ASEAN Gender Network launched. A two-tube, nested PCR detection method for AHPND bacteria. 9th Regional Grouper Hatchery Production Training Course. Developing an environmental monitoring system to strengthen fisheries and aquaculture in the Lower Mekong Basin. Asia-Pacific Regional Workshop on the Status of Aquatic Genetic Resources for Food and Agriculture.

In early 2013, Vibrio parahaemolyticus was discovered as the causative agent of acute hepatopancreatic necrosis disease (AHPND). Subjecting 4 arbitrarily selected V. parahaemolyticus isolates obtained from a single farm in November 2012 to the previously reported immersion challenge protocol, we confirmed that 3 caused high mortality at different rates accompanied by characteristic lesions of AHPND while 1 caused high mortality without such lesions. Virulence may be determined by mobile genetic elements such as plasmids or bacteriophages.