Bacterial diseases represent one of the major impediments to sustainable aquaculture. Routine screening for bacterial pathogens at various life stages of tilapia, carp and catfish a are important to minimise their introduction into production systems and before they can cause serious diseases and spread to new areas. During an abnormal mortality event, routine sampling for bacteriology from moribund animals should take place rapidly as part of the disease diagnostic investigation. Bacteriology is the culture and identification of bacteria growing under specific conditions. WorldFish and partners developed this quick guide on fish bacteriology sampling. Standard bacteriology is a swab taken from the caudal or anterior kidney and inoculated onto agar (e.g. Tryptone soya agar) to screen for systemic bacterial infection. Additional swabs may be included in the presence of external or internal lesions/ulcers (e.g., eye, skin, mouth, liver, spleen, brain). A free online training course on bacteriology sampling is also available via Learn.ink.

Major pathogens of fish and other farmed aquatic animals include bacteria, fungi, viruses and parasites. These can lead to serious infectious diseases and losses representing one of the major limitation to sustainable aquaculture. Routine health checks for key priority pathogens of tilapia, carp and catfish—will vary with geographies—are important to minimize the risk of their introduction, transmission and spread. On suspicion of an unknown disease during abnormal mortalities, it is routine for the investigators to collect clinical samples from moribund fish for molecular and virology diagnostics. Molecular diagnostics are techniques used to amplify small DNA/RNA sequence(s) that are unique to a particular pathogen to ascertain their presence or absence. Virology is a branch of microbiology that study viruses and viral diseases. WorldFish and partners developed this quick fish sampling guide for molecular and virology diagnostics. Standard specimens for general molecular and virology health evaluation include kidney, liver, spleen, brain and gills. Other tissues may be collected.

During investigation of an abnormal mortality event, fish tissues (or biopsies) for histopathology analysis are collected for disease diagnosis. Histology consists in the preparation of thin, stained tissue sections for microscopic examination to study their structure and function. Histopathology is the study of disease and disease processes by looking at the change in the anatomy or anomalies from cells, tissues and organs as seen through a microscope. WorldFish and partners developed this quick fish-sampling guide for histology. Standard biopsy specimens for histological examination consists of fixed sections of brain, gill, heart, intestine, kidney, liver and spleen. Other tissues may be collected in the presence of external lesions/ulcers (e.g., eye, skin, muscle). A free online training course on histology sampling is also available via Learn.ink.

In this issue:

• Second High-Level Meeting on Aquaculture Transformation in Asia and the Pacific Region.
• Reported aquatic animal diseases in the Asia-Pacific region during the second quarter of 2023.
• Expert Workshop on Aquaculture Effluent Management.
• Larvi 2024: First announcement and call for papers.
• Tuskfish CMS 2.0.7 released.

In 2023, FAO and NACA initiated a consultation process on aquaculture effluent management in Asia and the Pacific in collaboration with NACA member governments. Experts across the region gathered information to assess the state of governance, advancements in technology and innovation in aquaculture effluent management. The consultative process culminated in an expert workshop, convened from 14 to 15 November, funded by FAO, to share views and national experiences on aquaculture effluent management.

In this issue:

• How welfare assessments of farmed white leg shrimp (Penaeus vannamei) can benefit the whole industry
• Thai Fish Project: A path towards a sustainable aquaculture
• Nationally-recognised ornamental fish breeder Kripan Sarkar - a man to remember
• Tilapia parvovirus disease: An emerging threat for the tilapia aquaculture industry
• Naihati fish seed market as state-of-the-art for sustainable support services to fish growers, buyers and traders
• NACA Newsletter

A second High Level Meeting on Aquaculture Transformation: A Call for Collective Action was held in Bangkok from 8-9 November 2023 to further facilitate regional collaboration towards the Blue Transformation vision. The purpose of the meeting was to review progress made after the First HLM and discuss specific actions and mechanisms needed to enhance regional cooperation and progress transformation at scale by 2030. The meeting discussed and reviewed a draft Action Guide for Aquaculture Transformation for Asia and the Pacific Region. A proposal to develop an Asia-Pacific Aquaculture Innovation and Investment Hub as a regional mechanism providing policy advice, monitoring, resource mobilisation, partnership and capacity building functions as a regional platform driving aquaculture transformation was also considered.

The Thai Fish Project began in 2019, and involves around 200 research members and multiple institutions in Thailand and Japan. The project aims to promote domestication and wise use of two Thai native aquatic species, namely the Asian sea bass Lates calcarifer and banana shrimp Penaeus merguiensis through increasing productivity, reducing the impact of infectious diseases, and preserving genetic resources. The project also encompasses several specific research topics to ensure that it comprehensively addresses the concerns on safeguarding the food security and enhancing the environmental sustainability as much as possible. This article describes the main research activities, outputs and future expectations of the Thai Fish Project.

Naihati fish seed market of West Bengal was established in 1976 and is the largest seed market in India. With the advent of induced breeding technology and hatcheries, fish seed is now available almost year-round, catering to the need of Indian farmers. Situated in North 24 Parganas, Naihati wholesale fish seed market is a well-known establishment that provides multifaceted services to stakeholders including fish seed growers, retailers, sellers, and mediators. This article describes the state of the market as aspects of its evolution over time.

In late 2022 tilapia parvovirus (TiPV) was found to be associated with mass mortalities of tilapia in Odisha, India, both individually and in co-infections with tilapia lake virus. Infections have also been reported from Thailand and China. Clinical signs include lethargy, scale loss, redness on the body with haemorrhages on the operculum, base of fins and ventral part, opaqueness of the eyes, swimming near the pond edge and loss of appetite before death. This article describes the epidemiology of TiPV disease including its diagnosis, transmission, prevention and control, and its potential impact on the tilapia aquaculture industry.