The striped spiny eel, Macrognathus pancalus, is economically significant but faces population decline due to anthropogenic factors. This study presents a comprehensive approach to breeding and seed production techniques for M. pancalus, conducted at the Regional Research Centre, ICAR-Central Institute of Freshwater Aquaculture, Rahara, India. The study describes broodstock management simulation of natural habitat to stimulate natural breeding. Techniques for hatching and larval rearing, management of early spawn, and precautions to prevent cannibalism are presented. Survival of 88% after one month was achieved. The breeding and seed production techniques provided are suitable for farmers to adapt, and will contribute to the conservation and utilisation of M. pancalus in sustainable aquaculture practices. The findings also highlight the economic potential of this species to provide a nutritious option for consumers at affordable prices.

This study explores the cultivation of the Indian spiny loach, Lepidocephalichthys thermalis, in indigenously designed poly-lined iron frame tanks as a sustainable solution. The article details the fabrication methods for these tanks, highlighting the use of rust-resistant iron and low-density polyethylene sheets.The study proposes a supplementary feed mixture and provides insights into water quality management to ensure optimal conditions. The study demonstrates the potential for achieving a net production level of 1 kg from 400 liters, offering economic viability for farmers. The modified tank-based rearing system is shown to be beneficial in hilly regions with limited land and water resources.

Quality biological sampling is a fundamental requirement for all kinds of work on diagnosing diseases and screening for pathogens. Fish sampling requires specific techniques and skills to collect good biological samples for accurate disease diagnosis. This package of practices developed by WorldFish and partners provides advice on fish sampling protocols for routine disease diagnostics and outbreak investigations in tilapia, carp and catfish production systems. These sampling protocols provide guidance and training to project team members, researchers, resident veterinarians, extension officers and farmers tasked with collecting quality biological samples for laboratory diagnosis whenever disease outbreaks occur on farms or hatcheries. A free online introductory course on fish sampling for disease diagnostics is also available via Learn.ink.

Infectious diseases represent one of the major challenges and limitation to sustainable aquaculture. They can have major impacts on animal welfare, livelihood of farmers and threaten the health of captive stocks and wild populations. Pathogens can spread through movement of live fish, fish products, water and shared equipment. Routine screenings of pathogens in aquatic animal production systems are important to minimize the risk of their introduction, transmission and spread. On suspicion of a disease during abnormal mortalities, investigation with collection of biological samples from moribund animals must take place rapidly for disease diagnostics. Quality biological sampling is a fundamental requirement for all kinds of disease diagnostic and pathogen screening work. WorldFish and partners developed this rapid guide on sampling materials required for fish disease diagnostics. It lists all the personal protective equipment, the data collection/recording/cleaning supplies and sampling materials: consumables, reagents, media and tools needed to conduct one of the six quick fish sampling guides for disease diagnostics: 1. Wet-mount sampling guide (for ectoparasites & fungi) 2. Fish & water Microbiome sampling guide 3. Blood sampling guide 4. Bacteriology sampling guide 5. Molecular and virology sampling guide 6. Histology sampling guide.

A free online course on foundations in fish disease sampling is also available via Learn.ink.

Parasitic and fungal infestations represent one of the major challenges to sustainable aquaculture. As part of routine health checks or during abnormal mortality events, screening for ectoparasites and fungi in tilapia, carp and catfish production systems is important to minimize the risk of their introduction, transmission and spread. WorldFish and partners developed this quick guide for ectoparasites and fungi assessment under a light compound microscope. Standard wet-mount specimens consist of gill biopsy (gill clip), fin biopsy (fin clip) and skin scraping (mucus smears). Additional smears may be included in the presence of external lesions/ulcers (eye, skin, mouth). A free online training course on wet mount sampling is also available via Learn.ink.